|Karen McDonald, PhD
Professor, Dept. Chemical Engineering
3008 Bainer Hall
Office Phone: (530) 752-8314
Faculty Director, UC Davis ADVANCE Program
1104 Surge III
Office Phone: (530) 752-6885
|Somen Nandi, PhD
Adjunct Professor, Dept. Chemical Engineering
3112 Bainer Hall
Office Phone: (916) 838-0918
Managing Director, Global Healthshare® Initiative
318A Life Sciences Building
Office Phone: (530) 752-3613
Hiroto received his Ph.D. degree in biological science from Nara Institute of Science and Technology, Japan. He works for a Japanese food company.He began working in the McDonald/Nandi lab in March 2019 as a visiting scholar.His current research is in clarifying the appropriate condition of expression and purification of a human therapeutic protein, Alpha-1-antitrypsin, using a transient expression system in Nicotiana benthamiana.
Dexter is a first year Ph.D. student in Chemical Engineering at UC Davis. He received his B.S. in Chemical Engineering from Columbia University, New York and his B.A. in Chemistry from University of Puget Sound, Washington through a Dual Degree Program. He is currently developing stable lines of transgenic lettuce, which express a parathyroid hormone fusion protein.
|Kirolos (Kiro) Kelada
Kirolos (Kiro) Kelada: Kiro received his B.S. in Chemical Engineering with a specialization in biomolecular engineering from the University of California, Irvine in 2018. He is currently working on developing Techno-Economic Analyses using SuperPro modelling.
Eric is a master’s degree student and began working in the McDonald/Nandi lab in January 2019. He is studying the use of Nicotiana benthamiana plant cell suspension cultures for the transient expression of useful proteins. Eric received a B.S.E. in chemical engineering from the University of Iowa.
|Kantharakorn (Korn) Macharoen
Korn received a bachelor’s degree in Biotechnology and a master’s degree in Chemical Engineering from Chulalongkorn University, Thailand. He was awarded a Royal Thai Government Scholarship to study a doctoral degree in Chemical Engineering emphasizing in biomanufacturing of therapeutic proteins. His graduate research focuses on improving the production of recombinant butyrylcholinesterase (rBChE) in transgenic rice cell suspension cultures using bioreactor process optimization and scaling-up the production of rBChE to a pilot scale.
Matt received his B.S. in Chemical Engineering from the University of Massachusetts, Amherst. He previously worked as a process engineer for Sanofi Genzyme. His current research focuses on the technoeconomic analysis of a plant-made antimicrobial for food safety and on developing a novel biologically-derived bioseparations platform.
David earned his Bachelor of Science degree in Chemical Engineering with a biomolecular focus from UCLA in 2012. He then worked as a validation engineer for PSC Biotech in Los Angeles before joining the McDonald group, completing projects at Baxter Bioscience and Neutrogena Corporation. His current research is in optimizing the expression and purification of a human therapeutic protein, Alpha-1-antitrypsin, using a transient expression system in Nicotiana benthamiana.
|Yongao (Mary) Xiong
Mary joins McDonald group as a Ph.D student after finishing her undergraduate study in chemical engineering at University of Washington. Her current research is focused on transient expression of a Fc-fusion bioscavenger (CMG2-Fc) in Nicotiana Benthamiana whole-plant through agrobacterium vacuum infiltration, and the effects of N-glycosylation on protein properties. She also studies protein-protein interaction utilizing real-time label-free techniques (Surface plasmon resonance and biolayer interferometry) to quantitatively characterize bioscavenger’s binding kinetics and affinity against its target.
Kevin is a first year PhD student in Chemical Engineering at the University of California, Davis. He works in the McDonald-Nandi lab, where he develops recombinant plant cell wall degrading enzymes within the scope of NASA's CUBES project. Kevin earned a BS in Chemical Engineering with minors in Electrical Engineering and Mathematical Sciences from Michigan Technological University.
Min Du is a Biochemical Engineering undergraduate student at UC Davis. He started working in the lab in September 2018, and he is currently serving as an undergraduate assistant under the mentorship of Kantharakorn (Korn) Macharoen on the topic of pilot scale production of rrBChE with 40L bioreactor.
Dylan is a Chemical Engineering undergraduate student at the University of California, Davis. He previously worked on designing and synthesizing peptide nucleic acid and peptides to block and disrupt beta-amyloid protein at Sacramento City College. Currently, he works on developing a novel hand-powered centrifuge design.
Brandon Gregersen is an undergraduate studying Chemical Engineering. He started working at the lab in June of 2019 on the hand powered centrifuge project under Matt McNulty for NASA's CUBES. He plans on learning as much as he can from working in the lab, and then applying it to school and industry.
Seongwon Jung is a Chemical Engineering undergraduate student at UC Davis. He started working in the McDonal-Nandi lab from April 2019. He is currently working on optimizing the production of recombinant butyrylcholinesterase(rBChE) in transgenic rice cell suspension cultures under Kantharakorn Macharoen.
Joshua McMillan is an undergraduate student studying Chemical Engineering at the University of California, Davis. He is assisting with the Techno-Economic Analysis of the production of Thaumatin under the mentorship of Kirolos Kelada
Former Lab Members
|Name||Former Position||Thesis/Dissertation Title||Current Position|
|Lucas Arzola||Post-doctoral Scholar + Graduate Student (PhD)||Transient Expression of Recombinant Therapeutic Proteins and Vaccines Using Agroinfiltration in Nicotiana benthamiana||Director of Operations
Puerto Rico Science and Technology Trust
|Kalimuthu Karuppanan||Post-doctoral Scholar||Post-doctoral Scholar
Oxford University, UK
|Jose Antonio Rocha-Valadez||Post-doctoral Scholar||Bayer CropScience
West Sacramento, CA
|Remi Shih||Post-doctoral Scholar|
|Salem Alkanaimish||Graduate Student (PhD)||Bioprocess Engineering Approaches for Production and Purification of Recombinant Plant Made Human Butyrylcholinesterase||Assistant Professor
University of Kuwait
|Elizabeth Anthony Gill||Graduate Student (PhD)||Evaluation of Agrobacteria-mediated Transient Expression of Cell Wall Degrading Enzymes as a Biological Pretreatment Process for Cellulosic Biofuel Production||Director of Grid Policy
Center for Energy Efficiency and Renewable Technologies
|Shivaun Archer||Graduate Student (PhD)||Effect of Light on the Production of Sulfolipids from Cyanobacteria||John and Jent Swanson Senior Lecturer
|Nishant Bhatia||Graduate Student (PhD)||Ribosome Inactivating Proteins from Trichosanthes kirilowii Cultures||Director, Technology and Business Development
Advent Engineering Services
|Ching I. Chen||Graduate Student (PhD)||Analysis and Modelling for the Oscillatory Behavior of Saccharomyces Cerevisia in a Continuous Bioreactor System||Director, Process Development and Manufacturing
San Diego, CA
|Michael Cooney||Graduate Student (PhD)||Multivariable, Dynamic Model Development for Pure Continuous Cultures of E. Coli and C. Utilis||Associate Researcher
Hawaii Natural Energy Institute
University of Hawaii at Manoa
|Jasmine Corbin||Graduate Student (PhD)||Process Development for the Production of Recombinant Butyrylcholinesterase in Metabolically-Regulated, Transgenic Rice Cell Suspension Cultures||Scientist, Cell Culture Process Development
|Seher Dagdeviren||Graduate Student (MS)||Sulfolipid Production from the Cyanobacterium Synechocystis sp. PCC 6803 in Fed-Batch Photobioreactors||QC Supervisor
|Corey Dodge||Graduate Student (PhD)||Production of Recombinant Human Gelatin in Transgenic Rice Cell Cultures||Senior Staff Scientist
San Diego, CA
|Sifti Duhra-Gill||Graduate Student (MS)||Transient Expression of an Anthrax Decoy Fusion Protein in Nicotiana benthamiana||Air Resources Board
|Iftikar Gohar||Graduate Student (MS)||Numerical Simulation of Natural Convection in Liquid Semiconductor Metals in Modified Bridgman Furnace||Van den Bergh Foods
|Annette Hagewiesche||Graduate Student (MS)||Plan II||Senior Engineer
South San Francisco, CA
|Bryce Hashimoto||Graduate Student (MS)||Development of a Transgenic Rice Cell Line for Production of Butyrylcholinesterase: A Protective Bioscavenger against Sarin||Sandia National Laboratories
Kansas City, MO
|Ting-Kuo Huang||Graduate Student (PhD)||Development of Plant Cell Suspension Cultures as a Bioproduction Platform of Recombinant Human Therapeutic Proteins||Senior Engineer
South San Francisco
|SangKyu Jung||Graduate Student (PhD)||Agrobacterium-Mediated Transient Expression of Plant Cell Wall Degrading Enzymes in Detached Sunflower Leaves||Assistant Professor, Biochemical Engineering
|Nathaniel Kingsbury||Graduate Student (PhD)||Selective Recovery from the Interstitial Spaces of Nicotiana species||Senior Engineer, Late Stage Development
Santa Monica, CA
|Yen Ko||Graduate Student (MS)||Cyanobacteria Photoproduction in a Stirred Reactor||Unknown|
|Rajesh Krishnan||Graduate Student (PhD)||The Expression, Localization and Characterization of Recombinant Trichosanthin, a Type I Ribosome-Inactivating Protein, in Transgenic Tobacco||Director, Biologics Process Development
|Ingrid Leth||Graduate Student (PhD)||Production of Agrobacterium tumefaciens for Large-Scale Transient Expression of Recombinant Proteins in Plants||Scientist I
|Ben Lindenmuth||Graduate Student (PhD)||In Planta Expression of Heterologous Cell Wall-Degrading Enzymes||Drug Substance Tech Lead
|Michael Lucas||Graduate Student (MS)||Production Manager
|Christine Owlett||Graduate Student (MS)||Therapeutics from Plant Cell Suspension Cultures||Deceased|
|Sinyoung Park||Graduate Student (PhD)||Mammalian Cell Respiration and O2 /CO 2 Transfer in High-density Perfusion Culture with Microsparge Oxygenation and On-line Mass Spectrometry||Clinical Research Coordinator
|Kirti Patel||Graduate Student (MS)||Plan II||President and CEO
Executive Board Director
|Michael Plesha||Graduate Student (PhD)||Development of a Viral Amplicon-Based Process for Production of Biopharmaceuticals in Plant Tissues||Principal Scientist, Pilot Plant Lead
|Kittipong Rattanaporn||Graduate Student (PhD)||Quantitative RNA Analysis and Roles of Viral RNA Silencing Suppressors in Transient Therapeutic Protein Production in Plant Tissues using a Viral Amplicon-Based System||Associate Dean for International Relations
Lecturer, Department of Biotechnology
Faculty of Agro-Industry
|Melody (Trexler) Schmidt||Graduate Student (PhD)||A Cyclical Semi-Continuous Process for Production of Heterologous Proteins in Metabolically Regulated Plant Cell Suspension Cultures||Senior Scientist, Recovery Sciences
South San Francisco, CA
|Sara Sukenik||Graduate Student (PhD)||Transient Expression of an Anthrax Decoy Fusion Protein in Glycoengineered Plant Cell Suspension Cultures|
|Masaru Shiratori||Graduate Student (PhD)||Studies of a TMV-Based Expression Vector in Plant Cell Cultures||Senior Engineer
South San Francisco, CA
|John Thorup||Graduate Student (MS)||Purification and Identification of Ribosome Inactivating Proteins from Plant Cell Cultures of Trichosanthes Kirilowii||Senior Manager, Process Development
Thousand Oaks, CA
|Tiffany Tu||Graduate Student (MS)||Scale-up Simulation of Cellulase Enzymes Production for Biofuel Application: Fungal, Transgenic, and Transient Production of Cellulase Enzymes||Engineer
|James Wong||Graduate Student (PhD)||Classification of Process Trends Based on Fuzzified Symbolic Representation and Hidden Markov Models||Senior Software Developer
San Leandro, CA
|Qing Xie||Graduate Student (MS)||Recombinant Alpha-1-Antitrypsin Production in Transgenic Tobacco Cells||Biocatalytic
South San Francisco, CA
|Greg Young||Graduate Student (PhD)||Thermal and Stress Analysis of Single Crystal Growth in a Horizontal Bridgman Furnace||Emeritus Professor and Department Chair, Chemical Engineering Department
San Jose State University
San Jose, CA
|Liz Zapalac||Graduate Student (MS)||Purification of Alpha-1-Antitrypsin using a Three-Step Chromatography Process - Factors Effecting Dynamic Binding Capacity, Production Rate and Costs||Senior Plant Engineer
|Rupak Goswami, PhD||Visiting Scholar|
|Pauline Famy||Visiting Scholar|
|Oscar A. Ortega-Rivera||Visiting Scholar|
|Alexandra Ramirez Celis||Visiting Scholar|
|Trenton Smith||Visiting Scholar|
Former undergraduate researchers:
Karen has supervised the following students in undergraduate research projects (ECH199, PUFF program, NSF REU, MORE program, McNair Scholars program, NSF LURE, NSF CREATE-REU, UCD GREAT etc.)
An asterisk (*) indicates that the student went on to graduate school
Kostyantyn (Stan) Luferov, Mark Johnson*, Darrell Lewis-Sandy, Sara Stokes, Monica Gomes, Richard Cheung, Caroline Crispino, Tuyen Do, Yvette Mangaladin, Anh Nguyen, Mondana Panymanian, Eunice Tan*, Ruth Chan (MORE), Arlene Adela, James Fred Johnson*, Michelle Bishop*, Rachael Balog, Dave Reichmuth*, Casey Bunker*, Cynthia Buenviaje*, Michael Costello, Nicole Dennis, Richard St. John*, Tracy Horsfield, Yea-Lian Huang, Linda Leong, Khan Nguyen, Katrina Meehan, David Mills*, Phuong Anh Nguyen, Bill Ristenpart* (PUFF), James Corpuz, Chris Humphrey, Michael Stoner*, Devin Eselius, Danielle Coutts, Shaelyn Hurst (McNair Scholar), Ruth Chan, Christine Owlett*, Shane Hall, Annie Chou (MORE), Megumi Noguchi, Tammy Adamson (LURE), Phillip Tuet, Jolie Wu, Mark Sanford, Andrew Tung, Diemchi Nguyen, Jennifer Mikovich, Jeff Luk*, Ming-Wai Chen, Debby Kolbeck, Sandra VanDusen, Kali Campbell, Jennifer Chen, Lo-Ming (Alison) Hong*, David Trombly*, Javier Lacayo, Nathan Parker, Drew Erlich, (Vista) Li, Tammy Chan, Fareed Rudy Mohammed, Quyen Truong*, Kaushal Parikh*, Stacey Cox*, Michael Cheung, Marisa Harano* (MORE), Yanto Yanto*, Javier Cardenas*, Sinath Prom*, Randy Cross, Yevgeniya Mulina, Jeremy Lee*, Joel Thomas*, Jeffrey Upton, Marissa Roush, Yuchen Gu, Christian Millsop, Helena Keller* (Michigan Technological University) Li Qian (Grace) Bai, Allen Reed, Dane Emmerson, Debika Mitra* (CREATE-REU, Georgia Tech), Anh (Annie) Tran, Junxing (Cici) Chen, Huang (Sunny) Yan, Anna Iris Correa Muler (CREATE-REU, University of Puerto Rico Mayaguez), Derrick Hicks* (Integrated Studies Honors Thesis, PUF award), Alexandra Moskaluk * (BioChE Honors Thesis, UC Davis Veterinary School), Thuy Thanh Le*, Jacob Partlow, George Correra, Jerrine Wong, Kaylee Thatcher, Jack Morel, Darwin Constantino, Vincent Reyes, Katie Mains (BioChE Honors), Mathew (Peter) Kedzierski (BioChE Honors), Anthony Tsai * (UC Irvine), Ben Chang (ChE Honors), Zachary Kyser, Aaron Hwong, Kevin Tom (BioChE Honors), Jessie Delzio (BioChE Honors), Jacqueline Kelly, Vuthithorn Chinthammit, Min Du.